Protein NMR Techniques

Volume 831 of the series Methods in Molecular Biology pp 197-218


NMR Studies of Protein–RNA Interactions

  • Carla A. TheimerAffiliated withDepartment of Chemistry, University at Albany SUNY Email author 
  • , Nakesha L. SmithAffiliated withDepartment of Chemistry, University at Albany SUNY
  • , May KhannaAffiliated withDepartment of Biochemistry and Molecular Biology, Indiana University School of Medicine

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This chapter describes the preparation of NMR quantities of RNA purified to single-nucleotide resolution for protein–RNA interaction studies. The protocol is easily modified to make nucleotide-specific isotopically labeled RNAs or uniformly labeled RNA fragments for ligation to generate segmentally labeled RNAs.

Key words

In vitro transcription Single-nucleotide resolution RNA synthesis Protein–RNA interactions RNA purification Isotopic labeling