Protocol

Chromatin Remodeling

Volume 833 of the series Methods in Molecular Biology pp 1-14

Date:

Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library

  • Junbiao DaiAffiliated withSchool of Life Sciences, Tsinghua University
  • , Jef D. BoekeAffiliated withHigh Throughput Biology Center, Johns Hopkins University School of Medicine Email author 

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Abstract

A mutant library consisting of hundreds of designed point and deletion mutants in the genes encoding Saccharomyces cerevisiae histones H3 and H4 is described. Incorporation of this library into a suitably engineered yeast strain (e.g., bearing a reporter of interest), and the validation of individual library members is described in detail.

Key words

Histones modification Chromatin remodeling High-throughput mutagenesis