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Psychiatric Disorders

Volume 829 of the series Methods in Molecular Biology pp 329-348

Date:

Methods in Tobacco Abuse: Proteomic Changes Following Second-Hand Smoke Exposure

  • Joy Guingab-CagmatAffiliated withDepartment of Psychiatry, Center for Neuroproteomics and Biomarkers Research at the Evelyn F. and William L. McKnight Brain Institute
  • , Rayna M. BauzoAffiliated withDepartment of Psychiatry, College of Medicine, McKnight Brain Institute, University of Florida
  • , Adrie W. BruijnzeelAffiliated withDepartment of Psychiatry, College of Medicine, McKnight Brain Institute, University of Florida
  • , Kevin K. WangAffiliated withDepartments of Psychiatry, Center for Neuroproteomics and Biomarkers Research at the Evelyn F. and William L. McKnight Brain Institute, University of Florida
  • , Mark S. GoldAffiliated withDepartments of Psychiatry, Neuroscience, and Community Health & Family Medicine, Center for Neuroproteomics and Biomarkers Research at the Evelyn F. and William L. McKnight Brain Institute, University of Florida
  • , Firas H. KobeissyAffiliated withDivision of Addiction Medicine, Department of Psychiatry, Center for Neuroproteomics and Biomarkers Research at the Evelyn F. and William L. McKnight Brain Institute, University of Florida Email author 

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Abstract

Smoking is one of the leading preventable causes of disease, disability, and death in the USA and leads to more than 400,000 preventable deaths per year. Nicotine is the major alkaloid present in tobacco smoke, and many of the negative effects of smoking are attributed to nicotine. Nicotine is not only the addictive component of tobacco smoke, but also highly associated with carcinogenesis and induces oxidative stress. Furthermore, the administration of nicotine via subcutaneous mini-osmotic pumps or by injection is an established method in preclinical studies for this area of research. Thus, preclinical research on the negative effects of tobacco smoke and tobacco addiction has focused primarily on the effects of nicotine. However, there are over 4,500 components found in tobacco smoke, many of which are highly toxic. Other components may also contribute to the addictive properties of tobacco smoke. Furthermore, the negative effects of tobacco smoke are not isolated to the smoker but can have negative effects to those exposed to the secondhand smoke (SHS) stream. SHS exposure is the third leading cause of preventable death. Approximately 38,000 deaths per year are attributed to SHS exposure in the USA. SHS exposure increases the risk of heart disease by approximately 30% and is associated with increased risk of stroke, cancer, type II diabetes, as well as pulmonary disease. Thus, methods of administering tobacco smoke in a controlled environment will further our understanding of tobacco addiction and the role tobacco smoke in other disease states. Moreover, combining smoke exposure with proteomics can lead to the discovery of biomarkers that can be potentially useful tools in screening, early diagnosis, prevention, and treatment of diseases caused by SHS.

Key words

Tobacco Secondhand smoke Proteomics SHS Oxidative stress Addiction Toxicity