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Recombinant Gene Expression

Volume 824 of the series Methods in Molecular Biology pp 451-465

Date:

Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae

  • Sylvie Le BorgneAffiliated withDepartamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa Email author 

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Abstract

Genetic engineering has been successfully applied to Saccharomyces cerevisiae laboratory strains for different purposes: extension of substrate range, improvement of productivity and yield, elimination of by-products, improvement of process performance and cellular properties, and extension of product range. The potential of genetically engineered yeasts for the massive production of biofuels as bioethanol and other nonfuel products from renewable resources as lignocellulosic biomass hydrolysates has been recognized. For such applications, robust industrial strains of S. cerevisiae have to be used. Here, some relevant genetic and genomic characteristics of industrial strains are discussed in relation to the problematic of the genetic engineering of such strains. General molecular tools applicable to the manipulation of S. cerevisiae industrial strains are presented and examples of genetically engineered industrial strains developed for the production of bioethanol from lignocellulosic biomass are given.

Key words

Saccharomyces cerevisiae Industrial strain Adaptation Ethanol production Pentose fermentation Chromosomal integration Lignocellulosic biomass