Protocol

Bone Research Protocols

Volume 816 of the series Methods in Molecular Biology pp 103-117

Date:

Rodent Osteoclast Cultures

  • Isabel R. OrrissAffiliated withDepartment of Cell and Developmental Biology, University College London Email author 
  • , Timothy R. ArnettAffiliated withDepartment of Cell and Developmental Biology, University College London

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Abstract

This chapter describes quantitative methods for isolating and culturing rodent osteoclasts on dentine, a bone-like, resorbable substrate. These techniques generate relatively large numbers of osteoclasts and allow the key processes of osteoclast formation and activation to be studied independently. A special focus will be on the role of extracellular pH, a critical factor in the control of osteoclast function.

Key words

Osteoclast Resorption pH Bone Dentine