Protocol

Bone Research Protocols

Volume 816 of the series Methods in Molecular Biology pp 31-41

Date:

Rat Osteoblast Cultures

  • Isabel R. OrrissAffiliated withDepartment of Cell and Developmental Biology, University College London Email author 
  • , Sarah E. B. TaylorAffiliated withDepartment of Cell and Developmental Biology, University of Lancaster
  • , Timothy R. ArnettAffiliated withDepartment of Cell and Developmental Biology, University College London

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Abstract

This chapter describes the isolation, culture and staining of primary osteoblasts from the calvaria and long bones of neonatal rats. The key advantages of this assay are that it allows direct measurement of bone matrix deposition and mineralisation, as well as yielding good quantities of osteoblasts at defined stages of differentiation for molecular and histological analysis. A special focus of this chapter is on the role of β-glycerophosphate in cell-mediated mineralisation in these cultures.

Key words

Osteoblast Bone formation Mineralisation pH