Seed Dormancy

Volume 773 of the series Methods in Molecular Biology pp 151-164


Isolation of Genetic Material from Arabidopsis Seeds

  • Urszula PiskurewiczAffiliated withDépartement de Biologie Végétale, Université de Genève
  • , Luis Lopez-MolinaAffiliated withDépartement de Biologie Végétale, Université de Genève Email author 

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Here, we describe a series of methods suitable for the reproducible and abundant isolation of total RNA, genomic DNA, and total protein from dry or imbibed Arabidopsis seeds. The resulting material is suitable for most standard molecular biology procedures.

Key words

Seed germination RNA isolation DNA isolation Protein isolation Protein transfer to membrane cDNA synthesis RT-PCR Microarray analysis Sodium bisulfite sequencing Radioactive in vivo labeling Immunopurification