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Cell Migration

Volume 769 of the series Methods in Molecular Biology pp 233-247

Date:

Analysis of Cell Migration Using Caenorhabditis elegans as a Model System

  • Ming-Ching Wong
  • , Maria Martynovsky
  • , Jean E. SchwarzbauerAffiliated withDepartment of Molecular Biology, Princeton University Email author 

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Abstract

The nematode Caenorhabditis elegans is an excellent model system in which to study long-distance cell migration in vivo. This chapter describes methods used to study a subset of migratory cells in the hermaphrodite nematode, the distal tip cells. These methods take advantage of the organism’s transparent body and the expression of green fluorescent protein to observe cell migration and behavior. Additionally, the availability of nematode mutants and gene knockdown techniques that affect cell migration allow the analysis and comparison of wild-type and aberrant migratory paths. Methods for nematode growth and maintenance, strain acquisition, observation and live imaging, gene knockdown, and analysis of cell migration defects are covered.

Key words

C. elegans Cell migration Mutants RNAi Distal tip cells Live imaging Green fluorescent protein