Protocol

Human Pluripotent Stem Cells

Volume 767 of the series Methods in Molecular Biology pp 283-295

Date:

Human Pluripotent Stem Cells: The Development of High-Content Screening Strategies

  • Sean P. ShermanAffiliated withLab of Dr. April D. Pyle, Molecular Biology Institute, University of California, Los Angeles
  • , Jackelyn A. AlvaAffiliated withLab of Dr. April D. Pyle, Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles
  • , Kaushali Thakore-ShahAffiliated withLab of Dr. April D. Pyle, Molecular Biology Institute, University of California, Los Angeles
  • , April D. PyleAffiliated withMolecular Biology Institute, Department of Microbiology, Immunology, and Molecular Genetics, Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA, University of California, Los Angeles Email author 

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Abstract

High-content screening (HCS) permits simultaneous observation and analysis of multiple cellular variables including cell morphology, survival, and differentiation in live cells at the single-cell level, at the level of the culture well, and across the entire culture. By combining high-throughput technologies such as robotics, chemical libraries, and automated high-resolution microscopy, scientists are able to evaluate a much broader array of experimental conditions than can be studied using conventional cell biological techniques that study fewer parameters at any one time. Thus, HCS assays provide a means to vastly improve our basic understanding of stem cell biology. We have developed a HCS assay that allows the study of the effects of hundreds of small molecules in parallel. The protocol described in this chapter was developed to assess the effects of small molecules on the survival, proliferation, and expression of pluripotent markers following single-cell dissociation of human embryonic stem cells, but can be applied to the study of other types of stem cells including induced pluripotent stem cells. A detailed protocol for the setup of HCS assays and the parameters used to identify chemical modifiers of survival in human pluripotent stem cells, as well as secondary assays used to validate the small-molecule “hits” obtained during the high-content screen, are described.

Key words

human embryonic stem cells pluripotent stem cells high-content screening chemical genomics cell fate survival differentiation small molecules HCS