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Yeast Systems Biology

Volume 759 of the series Methods in Molecular Biology pp 87-106

Date:

Enrichment of Unstable Non-coding RNAs and Their Genome-Wide Identification

  • Helen NeilAffiliated withUnité de Génétique des Interactions Macromoléculaires, Institut Pasteur, CNRS, URA2171
  • , Alain JacquierAffiliated withUnité de Génétique des Interactions Macromoléculaires, Institut Pasteur, CNRS, URA2171 Email author 

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Abstract

Cryptic unstable transcripts (CUTs) have been recently described as a major class of non-coding RNAs. These transcripts are, however, extremely unstable in normal cells and their analyzes pose specific technical problems. In this chapter, after a brief introduction discussing general aspects associated with the analysis of non-coding RNAs, we provide details of methods to enrich, map, and quantify this unconventional class of transcripts.

Key words

Non-coding RNAs CUTs RNPs affinity purification 3′ Long-SAGE deep sequencing RNA-Seq