DNA Recombination

Volume 745 of the series Methods in Molecular Biology pp 523-536


Cell Biology of Homologous Recombination in Yeast

  • Nadine Eckert-BouletAffiliated withDepartment of Biology, University of Copenhagen
  • , Rodney RothsteinAffiliated withDepartment of Genetics and Development, Columbia University Medical Center
  • , Michael LisbyAffiliated withDepartment of Biology, University of Copenhagen Email author 

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Homologous recombination is an important pathway for error-free repair of DNA lesions, such as single- and double-strand breaks, and for rescue of collapsed replication forks. Here, we describe protocols for live cell imaging of single-lesion recombination events in the yeast Saccharomyces cerevisiae using fluorescence microscopy.

Key words

Homologous recombination fluorescence microscopy DNA damage DNA double-strand break repair