Protocol

Cystic Fibrosis

Volume 742 of the series Methods in Molecular Biology pp 173-185

Date:

Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells

  • Sophie CrespinAffiliated withLaboratory of Clinical Investigation III, Faculty of Medicine, Department of Pediatrics, Geneva University Hospitals and University of Geneva, Foundation for Medical Research
  • , Marc BacchettaAffiliated withLaboratory of Clinical Investigation III, Faculty of Medicine, Department of Pediatrics, Geneva University Hospitals and University of Geneva, Foundation for Medical Research
  • , Song HuangAffiliated withEpithelix, SàRL
  • , Tecla DudezAffiliated withLaboratory of Clinical Investigation III, Faculty of Medicine, Department of Pediatrics, Geneva University Hospitals and University of Geneva, Foundation for Medical Research
  • , Ludovic WiszniewskiAffiliated withEpithelix, SàRL
  • , Marc ChansonAffiliated withLaboratory of Clinical Investigation III, Faculty of Medicine, Department of Pediatrics, Geneva University Hospitals and University of Geneva, Foundation for Medical Research Email author 

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Abstract

One of the main functions of the airway mucosa is to maintain a mechanical barrier at the air–surface interface and to protect the respiratory tract from external injuries. Differentiation of human airway epithelial cells (hAECs) to polarized airway mucosa can be reproduced in vitro by culturing the cells on microporous membrane at the air–liquid interface. Here, we describe approaches to study differentiation as well as repair of the hAECs by using a commercially available airway cell culture model called MucilAir™.

Key words

Human airway epithelial cell culture MucilAir™ differentiation wounding techniques repair cystic fibrosis