Protocol

Neuroproteomics

Volume 57 of the series Neuromethods pp 13-26

Date:

Dissection of Rodent Brain Regions

  • Sabine SpijkerAffiliated withDepartment of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, VU University Email author 

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Abstract

Dissection of brain tissue is an important step in sample preparation for (subcellular) proteomics studies. In this chapter, brain removal and separate dissection of multiple brain regions from a single brain are described in step-by-step protocol. This concerns dissection from fresh or frozen tissue of cerebellum, hippocampus, prefrontal cortex, and striatum.

Key words

Dissection Mouse Brain Hippocampus Cerebellum Cortex