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Protein Microarray for Disease Analysis

Volume 723 of the series Methods in Molecular Biology pp 81-104

Date:

Recombinant Antigen Microarrays for Serum/Plasma Antibody Detection

  • Persis P. WadiaAffiliated withDepartment of Medicine, Blood and Marrow Transplantation Division, Stanford University
  • , Bita SahafAffiliated withDepartment of Medicine, Blood and Marrow Transplantation Division, Stanford University
  • , David B. MiklosAffiliated withDepartment of Medicine, Blood and Marrow Transplantation Division, Stanford University Email author 

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Abstract

Recombinant antigen arrays represent a new frontier in parallel analysis of multiple immune response profiles requiring only minute blood samples. In this article, we review the benefits and pitfalls of recombinant antigen microarrays developed for multiplexed antibody quantification. In particular, we describe the development of antigen arrays presenting a set of Y chromosome-encoded antigens, called H-Y antigens. These H-Y antigens are immunologically recognized as minor histocompatibility antigens (mHA) following allogeneic blood and organ transplantation. Clinically relevant B-cell responses against H-Y antigens have been demonstrated in male patients receiving female hematopoietic stem cell grafts and are associated with chronic graft versus host development. This chapter discusses our recombinant antigen microarray methods to measure these clinically relevant allo-antibodies.

Key words

H-Y proteins Antibodies Plasma Recombinant antigen microarrays Minor histocompatibility antigens