Protein Microarray for Disease Analysis

Volume 723 of the series Methods in Molecular Biology pp 15-28


Analysis of Serum Protein Glycosylation with Antibody–Lectin Microarray for High-Throughput Biomarker Screening

  • Chen LiAffiliated withDepartment of Chemistry, The University of Michigan
  • , David M. LubmanAffiliated withDepartment of Chemistry, Comprehensive Cancer Center, The University of MichiganDepartment of Surgery, The University of Michigan Medical Center Email author 

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The complexity of carbohydrate structures and their derivatives makes the study of the glycome a challenging subset of proteomic research. The microarray platform has become an essential tool to characterize glycan structure and to study glycosylation-related biological interactions, by using probes as a means to interrogate the spotted or captured glycosylated molecules on the arrays. The high-throughput and reproducible nature of microarray platforms have been highlighted by their extensive applications in the field of biomarker validation, where a large number of samples must be analyzed multiple times. This chapter presents an antibody–lectin microarray approach, which allows the efficient, multiplexed study of the glycosylation of multiple individual proteins from complex mixtures with both fluorescence labeling detection and label-free detection based on mass spectrometry.

Key words

Microarray Antibody Glycoprotein Biomarker Serum Lectin MALDI Mass spectrometry