Protocol

Magnetic Resonance Neuroimaging

Volume 711 of the series Methods in Molecular Biology pp 349-361

Date:

MRI Phenotyping of Genetically Altered Mice

  • Jason P. LerchAffiliated withThe Mouse Imaging Centre, Hospital for Sick ChildrenDepartment of Medical Biophysics, University of Toronto Email author 
  • , John G. SledAffiliated withDepartment of Medical Biophysics, The Mouse Imaging Centre, Hospital for Sick ChildrenUniversity of Toronto
  • , R. Mark HenkelmanAffiliated withThe Mouse Imaging Centre, Hospital for Sick ChildrenDepartment of Medical Biophysics, University of Toronto

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The laboratory mouse, with its genetic similarity to humans and rich set of tools for manipulating its genome, has emerged as one of the key models for experimental investigation of the genotype/phenotype relationships in mammals. Recent innovations have made MRI an increasingly popular tool for examining the phenotype of genetically altered mice. Advances in field strengths, mouse handling, image analysis and statistics have contributed greatly in this regard. In this chapter, we illustrate the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI combined with advanced image analysis techniques and statistics.

Key words

Magnetic resonance imaging phenotyping mouse models genetics