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Metabolic Profiling

Volume 708 of the series Methods in Molecular Biology pp 147-157

Date:

Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry

  • Rajan S. KombuAffiliated withDepartment of Nutrition, Mouse Metabolic Phenotyping Center, Case Western Reserve University
  • , Henri BrunengraberAffiliated withDepartment of Nutrition, Mouse Metabolic Phenotyping Center, Case Western Reserve University
  • , Michelle A. PuchowiczAffiliated withDepartment of Nutrition, Mouse Metabolic Phenotyping Center, Case Western Reserve University Email author 

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Abstract

Researchers view analysis of the citric acid cycle (CAC) intermediates as a metabolomic approach to identifying unexpected correlations between apparently related and unrelated pathways of metabolism. Relationships of the CAC intermediates, as measured by their concentrations and relative ratios, offer useful information to understanding interrelationships between the CAC and metabolic pathways under various physiological and pathological conditions. This chapter presents a relatively simple method that is sensitive for simultaneously measuring concentrations of CAC intermediates (relative and absolute) and other related intermediates of energy metabolism using gas chromatography-mass spectrometry.

Key words

Citric acid cycle CAC intermediates GC-MS metabolomics mass spectrometry