Protocol

Adipose-Derived Stem Cells

Volume 702 of the series Methods in Molecular Biology pp 443-451

Date:

Differentiation of Adipose-Derived Stem Cells for Tendon Repair

  • A. Cagri UysalAffiliated withDepartment of Plastic and Reconstructive Surgery, Faculty of Medicine, Baskent University
  • , Hiroshi MizunoAffiliated withDepartment of Plastic and Reconstructive Surgery, School of Medicine, Juntendo University

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The goal of primary tendon repair is to increase tensile strength at the time of mobilization. Tendon repair and regeneration using mesenchymal stem cells have been described in several studies; however, the use of adipose derived stem cells (ASCs) for tendon repair has only recently been considered. In order to establish a suitable experimental model for the primary tendon repair using ASCs, this chapter describes the detailed methods for: (1) isolating stem cells from adipose tissue, (2) generation of a primary tendon injury and repair model, (3) evaluating functional restoration by measuring tensile strength, and (4) investigating the mechanisms involved in ASC-mediated tendon healing by histological and immunohistochemical analyses. Topical administration of ASCs to the site of injury accelerates tendon repair, as exhibited by a significant increase in tensile strength, direct differentiation of ASCs toward tenocytes and endothelial cells, and increases in angiogenic growth factors. These findings suggest that ASCs may have a positive effect on primary tendon repair and may be useful for future cell-based therapy.

Key words

Wound healing Adipose-derived stem cells Tendon Wound healing Tensile strength Angiogenesis Growth factors