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Adipose-Derived Stem Cells

Volume 702 of the series Methods in Molecular Biology pp 193-200

Date:

Adipogenic Differentiation of Adipose-Derived Stem Cells

  • Gang YuAffiliated withStem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System
  • , Z. Elizabeth FloydAffiliated withUbiquitin Laboratory, Pennington Biomedical Research Center, Louisiana State University System
  • , Xiying WuAffiliated withStem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System
  • , Teddi HebertAffiliated withStem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System
  • , Yuan-Di C. HalvorsenAffiliated withCenter for Computational and Integrative Biology, Harvard Medical School
  • , Benjamin M. BuehrerAffiliated withZenBio, Inc., Research
  • , Jeffrey M. GimbleAffiliated withStem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System Email author 

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Abstract

The primary physiological function of adipose-derived stem cells (ASCs) is to differentiate into adipose tissue. It is now possible to isolate, expand, and cryopreserve ASC from adipose depots of many animal species. These ASC can be induced to undergo adipogenic differentiation in vitro by exposure to a cocktail of chemical agents or inductive growth factors. The current chapter describes methods to induce adipogenesis and to quantify this differentiation process in vitro.

Key words

Adipose-derived stem cells Adipogenesis Basic fibroblast growth factor Bone morphogenetic protein CCAAT/enhancer binding protein α Cyclic adenosine monophosphate Epidermal growth factor Glucocorticoid receptor Indomethacin Insulin Isobutylmethylxanthine Peroxisome proliferator-activated receptor γ2 Rabbit serum