Mesenchymal Stem Cell Assays and Applications

Volume 698 of the series Methods in Molecular Biology pp 201-214


Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells

  • Deepak M. Gupta
  • , Nicholas J. Panetta
  • , Michael T. LongakerAffiliated withHagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford University School of Medicine Email author 

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A comprehensive knowledge of the molecular biology underlying osteogenic differentiation in a controlled, laboratory setting may promise optimization of future cell-based tissue engineering strategies for clinical problems. The scope of this review encompasses a discussion of the methodology utilized to perform such studies. Our laboratory routinely performs both in vitro and in vivo assays underlying osteogenic differentiation, and the widespread use of singular methodology across multiple investigators and institutions promises great advancements for the skeletal tissue engineering community.

Key words

Osteogenic differentiation Skeletal tissue engineering Regeneration Mesenchymal stromal cells Bone marrow Adipose derived stromal cells Alizarin red