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Data Mining in Proteomics

Volume 696 of the series Methods in Molecular Biology pp 369-377

Date:

LC/MS Data Processing for Label-Free Quantitative Analysis

  • Patricia M. PalagiAffiliated withProteome Informatics Group, Swiss Institute of Bioinformatics
  • , Markus MüllerAffiliated withProteome Informatics Group, Swiss Institute of Bioinformatics
  • , Daniel WaltherAffiliated withProteome Informatics Group, Swiss Institute of Bioinformatics
  • , Frédérique LisacekAffiliated withProteome Informatics Group, Swiss Institute of Bioinformatics

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Abstract

In this chapter, we describe the use of SuperHirn and MSight, two complementary tools developed to the processing of label-free LC/MS data in view of the quantitation of proteomics samples. While MSight is mainly dedicated to the visualisation and navigation into LC/MS data, SuperHirn is specialised in peak detection, normalisation and alignment of LC/MS runs. These two tools can be used in a complementary way and one of the possible usages is described here.