Protocol

Data Mining in Proteomics

Volume 696 of the series Methods in Molecular Biology pp 3-26

Date:

Instruments and Methods in Proteomics

  • Caroline MayAffiliated withDepartment of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum
  • , Frederic BrosseronAffiliated withDepartment of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum
  • , Piotr ChartowskiAffiliated withDepartment of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum
  • , Cornelia SchumbrutzkiAffiliated withDepartment of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum
  • , Bodo SchoenebeckAffiliated withDepartment of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum Email author 
  • , Katrin MarcusAffiliated withDepartment of Functional Proteomics, Medizinisches Proteom-Center, Ruhr-Universität Bochum

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Abstract

In the past decade, major developments in instrumentation and methodology have been achieved in proteomics. For proteome investigations of complex biological samples derived from cell cultures, tissues, or whole organisms, several techniques are state of the art. Especially, many improvements have been undertaken to quantify differences in protein expression between samples from, e.g., treated vs. untreated cells and healthy vs. control patients. In this review, we give a brief insight into the main techniques, including gel-based protein separation techniques, and the growing field of mass spectrometry.