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Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

Volume 690 of the series Methods in Molecular Biology pp 301-314

Date:

Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent

  • Andre ObenausAffiliated withNon-Invasive Imaging Laboratory, Department of Biophysics and Bioengineering, Departments of Radiation Medicine, Pediatrics, and Radiology, School of Medicine, School of Science and Technology, Loma Linda University Email author 
  • , Pedro HayesAffiliated withNon-Invasive Imaging Laboratory, Departments of Radiation Medicine, Pediatrics, and Radiology, Department of Biophysics and Bioengineering, School of Medicine, School of Science and Technology, Loma Linda University

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Abstract

Advances in stem therapy, scaffolds, and therapeutic biomolecules are accelerating bone repair research, and model systems are required to test new methods and concepts. The drill hole defect is one such model and is used to study a variety of bone defects and potential therapies designed to repair these injuries. We detail the methodologies required to successfully generate and evaluate drill hole defects. Although performing a successful drill hole defect requires patience and dexterity, investing the time to perfect the technique will provide ample opportunity for the researcher to expand his/her particular research interests. Mastering this technique will allow testing of stem cell therapies, novel scaffold designs, and biomolecules that can be used for clinical translation.

Key words

Drill hole defect Surgery Stereotaxic Magnetic resonance imaging Computerized tomography