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Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

Volume 690 of the series Methods in Molecular Biology pp 285-300

Date:

Analysis of Glycosaminoglycans in Stem Cell Glycomics

  • Boyangzi LiAffiliated withDepartment of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
  • , Haiying LiuAffiliated withDepartment of Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
  • , Zhenqing ZhangAffiliated withDepartment of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
  • , Hope E. StansfieldAffiliated withDepartments of Chemistry and Chemical Biology and Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
  • , Jonathan S. DordickAffiliated withDepartments of Biology and Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute
  • , Robert J. LinhardtAffiliated withDepartment of Chemistry and Chemical Biology and Department of Biology and Department of Chemical and Biological Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute Email author 

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Abstract

Glycosaminoglycans (GAGs) play a critical role in the binding and activation of growth factors in cell signal transduction required for biological development. A glycomics approach can be used to examine GAG content, composition, and structure in stem cells in order to characterize their general differentiation. Specifically, this method may be used to evaluate chondrogenic differentiations by profiling for the GAG content of the differentiated cells. Here, embryonic-like teratocarcinoma cells, NCCIT, a developmentally pluripotent cell line, were used as a model for establishing GAG glycomic methods, but will be easily transferrable to embryonic stem cell cultures.

Key words

Glycosaminoglycans NCCIT cells Chondroitin sulfate Dermatan sulfate Heparin Heparan sulfate Purification Enzymatic digestion Disaccharide analysis LC-MS