Methods in Molecular Biology Volume 687, 2011, pp 193-205

The Many Faces of MLPA

Purchase on Springer.com

$49.95 / €39.95 / £34.95*

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Multiplex Ligation-dependent Probe Amplification (MLPA) is a PCR-based technique that was developed for identifying deletions and duplications in genomic DNA. The simplicity and sensitivity of this approach has led to it being implemented in many laboratories around the world. Since the original publication, there have been several variants of MLPA described, allowing the quantitative analysis of mRNA transcript levels, CpG methylation, complex genomic regions, and DNaseI hypersensitive sites. This chapter outlines the basic MLPA protocol, describes the different modifications and applications that have been published, and discusses the critical points during each of the steps.