Principles and Methods of Immunohistochemistry
- José A. Ramos-Vara
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Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in the characterization of normal and pathologic tissue and the reagents used. Pretreatments such as blocking steps for endogenous activities and antigen retrieval are included. Standard procedures on formalin-fixed, paraffin-embedded tissues as well as method standardization for new antibodies and troubleshooting are emphasized.
- Ramos-Vara, J.A., Kiupel, M., Baszler, T., Bliven, L., Brodersen, B., Chelack, B., Czub, S., Del Piero, F., Dial, S., Ehrhart, E.J., Graham, T., Manning, L., Paulsen, D., Valli, V.E., and West, K. (2008) Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories. J. Vet. Diagn. Invest. 20, 393–413.
- Ramos-Vara, J.A., and Beissenherz, M.E. (2000) Optimization of immunohistochemical methods using two different antigen retrieval methods on formalin-fixed, paraffin-embedded tissues: experience with 63 markers. J. Vet. Diagn. Invest. 12, 307–311.
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- Principles and Methods of Immunohistochemistry
- Book Title
- Drug Safety Evaluation
- Book Subtitle
- Methods and Protocols
- pp 83-96
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series Subtitle
- Methods and Protocols
- Series ISSN
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
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- Antigen retrieval
- Detection methods
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