Protocol

Computational Biology

Volume 673 of the series Methods in Molecular Biology pp 211-222

Date:

Protein Quantitation Using Mass Spectrometry

  • Guoan ZhangAffiliated withKimmel Center for Biology and Medicine at the Skirball Institute and Department of Pharmacology, New York University School of Medicine
  • , Beatrix M. UeberheideAffiliated withThe Rockefeller University
  • , Sofia WaldemarsonAffiliated withKimmel Center for Biology and Medicine at the Skirball Institute and Department of Pharmacology, New York University School of Medicine
  • , Sunnie MyungAffiliated withThe Rockefeller University
  • , Kelly MolloyAffiliated withThe Rockefeller University
  • , Jan ErikssonAffiliated withDepartment of Chemistry, Swedish University of Agricultural Sciences
  • , Brian T. ChaitAffiliated withThe Rockefeller University
  • , Thomas A. NeubertAffiliated withKimmel Center for Biology and Medicine at the Skirball Institute and Department of Pharmacology, New York University School of Medicine
  • , David FenyöAffiliated withThe Rockefeller University Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Mass spectrometry is a method of choice for quantifying low-abundance proteins and peptides in many biological studies. Here, we describe a range of computational aspects of protein and peptide quantitation, including methods for finding and integrating mass spectrometric peptide peaks, and detecting interference to obtain a robust measure of the amount of proteins present in samples.

Key words

Proteomics Quantitation Proteins Peptides Mass spectrometry