Protocol

Oral Biology

Volume 666 of the series Methods in Molecular Biology pp 87-102

Date:

Protocols to Study the Physiology of Oral Biofilms

  • José A. LemosAffiliated withDepartment of Microbiology and Immunology, University of Rochester Medical CenterCenter for Oral Biology, University of Rochester Medical Center
  • , Jacqueline AbranchesAffiliated withDepartment of Microbiology and Immunology, University of Rochester Medical CenterCenter for Oral Biology, University of Rochester Medical Center
  • , Hyun KooAffiliated withDepartment of Microbiology and Immunology, Center for Oral Biology, University of Rochester Medical CenterEastman Department of Dentistry, University of Rochester Medical Center
  • , Robert E. MarquisAffiliated withDepartment of Microbiology and Immunology, Center for Oral Biology, University of Rochester Medical Center
  • , Robert A. BurneAffiliated withDepartment of Oral Biology, University of Florida College of Dentistry

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Abstract

The oral cavity harbors several hundred different bacterial species that colonize both hard (teeth) and soft tissues, forming complex populations known as microbial biofilms. It is widely accepted that the phenotypic characteristics of bacteria grown in biofilms are substantially different from those grown in suspensions. Because biofilms are the natural habitat for the great majority of oral bacteria, including those contributing to oral diseases, a better understanding of the physiology of adherent populations is clearly needed to control oral microbes in health and disease. In this chapter, we use oral streptococci as examples for studying the physiology of oral biofilms.

Key words

Biofilm oral streptococci Streptococcus enzymatic assays stress production of polysaccharides