Protocol

Diagnostic Virology Protocols

Volume 665 of the series Methods in Molecular Biology pp 369-382

Date:

Detection of SARS Coronavirus

  • J. S. Malik PeirisAffiliated withState Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong
  • , Leo L. M. PoonAffiliated withState Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong Email author 

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Abstract

The emergence of severe acute respiratory syndrome (SARS) and its subsequent worldwide spread challenged the global public health community to confront a novel infectious disease. The infection is caused by a coronavirus of animal origin. In this epidemic, molecular detections of SARS coronavirus RNA were shown to be useful for the early diagnosis of SARS. Although this pathogen was eradicated in humans, SARS or SARS-like viruses might reemerge from animals or from laboratory incidents. In this chapter, we describe several polymerase chain reaction (PCR) protocols for detecting SARS coronaviruses. These assays were routinely used for clinical diagnosis during the SARS outbreak.

Key words

SARS coronavirus RT-PCR Molecular detection Clinical diagnosis