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Mass Spectrometry Imaging

Volume 656 of the series Methods in Molecular Biology pp 159-171

Date:

Laser Ablation Electrospray Ionization for Atmospheric Pressure Molecular Imaging Mass Spectrometry

  • Peter NemesAffiliated withDepartment of Chemistry, W. M. Keck Institute for Proteomics Technology and Applications, George Washington University
  • , Akos VertesAffiliated withDepartment of Chemistry, W. M. Keck Institute for Proteomics Technology and Applications, George Washington University

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Abstract

Laser ablation electrospray ionization (LAESI) is a novel method for the direct imaging of biological tissues by mass spectrometry. By performing ionization in the ambient environment, this technique enables in vivo studies with potential for single-cell analysis. A unique aspect of LAESI mass spectrometric imaging (MSI) is depth profiling that, in combination with lateral imaging, permits 3D molecular imaging for the first time under native conditions. With current lateral and depth resolutions of ∼100 and ∼40 μm, respectively, LAESI MSI helps to explore the molecular architecture of live tissues.

Key words

Mass spectrometry imaging ambient direct analysis depth profiling three-dimensional in vivo tissue imaging