Protocol

Mass Spectrometry Imaging

Volume 656 of the series Methods in Molecular Biology pp 363-383

Date:

Structurally Selective Imaging Mass Spectrometry by Imaging Ion Mobility-Mass Spectrometry

  • John A. McLeanAffiliated withDepartment of Chemistry, Vanderbilt Institute of Chemical Biology, and Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University
  • , Larissa S. FennAffiliated withDepartment of Chemistry, Vanderbilt Institute of Chemical Biology, and Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University
  • , Jeffrey R. EndersAffiliated withDepartment of Chemistry, Vanderbilt Institute of Chemical Biology, and Vanderbilt Institute for Integrative Biosystems Research and Education, Vanderbilt University

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Abstract

This chapter describes the utility of structurally based separations combined with imaging mass spectrometry (MS) by ion mobility-MS (IM-MS) approaches. The unique capabilities of combining rapid (μs-ms) IM separations with imaging MS are detailed for an audience ranging from new to potential practitioners in IM-MS technology. Importantly, imaging IM-MS provides the ability to rapidly separate and elucidate various types of endogenous and exogenous biomolecules (e.g., nucleotides, carbohydrates, peptides, and lipids), including isobaric species. Drift tube and traveling wave IM-MS instrumentation are described and specific protocols are presented for calculating ion–neutral collision cross sections (i.e., apparent ion surface area or structure) from experimentally obtained IM-MS data. Special emphasis is placed on the use of imaging IM-MS for the analysis of samples in life sciences research (e.g., thin tissue sections), including selective imaging for peptide/protein and lipid distributions. Future directions for rapid and multiplexed imaging IM-MS/MS are detailed.

Key words

Ion mobility ion mobility-mass spectrometry IM-MS imaging mass spectrometry IMS MSI imaging ion mobility-mass spectrometry structural separations MALDI IM-MS/MS