Protocol

Plant Secondary Metabolism Engineering

Volume 643 of the series Methods in Molecular Biology pp 77-93

Date:

Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength

  • Joyce Van EckAffiliated withThompson Institute for Plant Research, Cornell University
  • , Xiangjun ZhouAffiliated withUS Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Department of Plant Breeding and Genetics, Cornell University
  • , Shan LuAffiliated withState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University
  • , Li LiAffiliated withUS Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Cornell UniversityDepartment of Plant Breeding and Genetics, Cornell University

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Abstract

An increasing interest in carotenoids as nutritional sources of provitamin A and health-promoting compounds has prompted a significant effort in metabolic engineering of carotenoid content and composition in food crops. The strategy commonly used in plants is to increase the biosynthetic capacity by altering the carotenogenic enzyme activities. The recent isolation of the Or gene from a cauliflower orange mutant has brought a new endeavor for carotenoid enhancement by increasing the sink strength to sequester and store the synthesized carotenoids. Potato as one of the major staple crops usually accumulates low levels of carotenoids. In this chapter, we describe a detailed protocol for metabolic engineering of carotenoids in potato plants with the Or gene and the analysis of the Or transformants.

Key words

Cauliflower Or gene carotenoids chromoplasts transgenic potato sink strength