Protocol

The Urinary Proteome

Volume 641 of the series Methods in Molecular Biology pp 65-88

Date:

Different Sample Preparation and Detection Methods for Normal and Lung Cancer Urinary Proteome Analysis

  • Supachok Sinchaikul
  • , Payungsak TantipaiboonwongAffiliated withInstitute of Biological Chemistry, Department of Chemistry, Faculty of Scienc, Chiang Mai University
  • , Supawadee SriyamAffiliated withInstitute of Biological Chemistry and Genomics Research Center, Academia SinicaDepartment of Chemistry, Faculty of Science, Chiang Mai University
  • , Ching TzaoAffiliated withDivision of Thoracic Surgery, Tri-Service General Hospital, National Defense Medical Center
  • , Suree PhutrakulAffiliated withDepartment of Chemistry, Faculty of Science, Chiang Mai University
  • , Shui-Tein ChenAffiliated withInstitute of Biological Chemistry and Genomics Research Center, Academia SinicaInstitute of Biochemical Sciences, College of Life Science, National Taiwan University Email author 

* Final gross prices may vary according to local VAT.

Get Access

Abstract

The urinary proteome is known to be a valuable field of study related to human physiological functions because many components in urine provide an alternative to blood plasma as a potential source of disease biomarkers useful in clinical diagnosis and therapeutic application. Due to the variability and complexity of urine, sample preparation is very important for decreasing the dynamic range of components and isolating specific urinary proteins prior to analysis. We discuss many useful sample preparation methods in this chapter, including those of lung cancer urine samples. In addition, protein detection methods are also crucial in visualizing protein profiles and for quantification of protein content in urine samples from both normal donor and lung cancer patients. This chapter also provides alternative choices of urine sample preparation and detection methods for selective use in urinary proteome analysis and for identifying urinary protein markers in lung cancer and other diseases.

Key words

Urine Lung cancer Proteomics Sample preparation Detection