Protocol

Photodynamic Therapy

Volume 635 of the series Methods in Molecular Biology pp 155-173

Date:

Antimicrobial Photodynamic Inactivation and Photodynamic Therapy for Infections

  • Liyi HuangAffiliated withWellman Center for Photomedicine, Massachusetts General HospitalDepartment of Dermatology, Harvard Medical SchoolDepartment of Infectious Diseases, First Affiliated College and Hospital, Guangxi Medical University
  • , Tianhong DaiAffiliated withWellman Center for Photomedicine, Massachusetts General HospitalDepartment of Dermatology, Harvard Medical School
  • , Michael R. HamblinAffiliated withWellman Center for Photomedicine, Massachusetts General HospitalDepartment of Dermatology, Harvard Medical SchoolHarvard-MIT Division of Health Sciences and Technology

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Abstract

Photodynamic therapy (PDT) was initially discovered over 100 years ago by its ability to kill microorganisms, but its use to treat infections clinically has not been much developed. However, the present relentless increase in antibiotic resistance worldwide and the emergence of strains that are resistant to all known antibiotics has stimulated research into novel antimicrobial strategies such as PDT that are thought to be unlikely to lead to the development of resistance. In this chapter we will cover the use of PDT to kill pathogenic microbial cells in vitro and describe a mouse model of localized infection and its treatment by PDT without causing excessive damage to the host tissue.

Key words

Bacteria fungus microbiology colony-forming units Photorhabdus luminescens luciferase bioluminescence imaging mouse model of localized infection antibiotic wound healing