Purification of Antimicrobial Peptides from Human Skin
- Jens-M. Schröder
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Human skin is a rich source of human antimicrobial peptides. Its cellular source is the keratinocyte, which terminally differentiates in the uppermost parts of the skin, eventually forming the stratum corneum, the horny layer. The easy availability of human stratum corneum makes it possible to identify and characterize human antimicrobial peptides with a biochemical approach. Moreover, the availability of lesional scales of patients with psoriasis, an inflammatory skin disease, allows the identification of human-inducible peptide antibiotics, which are absent in healthy skin. With this strategy, the beta-defensins hBD-2 and hBD-3, RNase-7 as well as psoriasin/S100A7 have been discovered as human antimicrobial peptides and proteins. A detailed description of the strategies and methods is presented, which allowed a successful identification and characterization of human antimicrobial peptides. We used various HPLC techniques, combined with antimicrobial testing as read-out system. In parallel, SDS-PAGE analyses as well as electrospray ionization mass spectrometry were used for further biochemical characterization as well as purity assessment.
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- Purification of Antimicrobial Peptides from Human Skin
- Book Title
- Antimicrobial Peptides
- Book Subtitle
- Methods and Protocols
- Book Part
- Section I
- pp 15-30
- Print ISBN
- Online ISBN
- Series Title
- Methods in Molecular Biology
- Series Volume
- Series Subtitle
- Methods and Protocols
- Series ISSN
- Humana Press
- Copyright Holder
- Springer Science+Business Media, LLC
- Additional Links
- Antimicrobial peptides
- stratum corneum
- electrospray ionization mass spectrometry
- high-performance liquid chromatography
- Industry Sectors
- Editor Affiliations
- ID1. SpiderBiotech S.r.l.
- ID2. Dipto. Scienze e Tecnologie Biomediche, Università di Cagliari
- Jens-M. Schröder (1)
- Author Affiliations
- 1. Clinical Research Unit “Cutaneous Inflammation”, Department of Dermatology, University Hospital Schleswig-Holstein, Kiel, Germany
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