Protocol

Peptidomics

Volume 615 of the series Methods in Molecular Biology pp 227-246

Date:

Quantitative Neuroproteomics of the Synapse

  • Dinah Lee Ramos-OrtolazaAffiliated withDepartment of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine
  • , Ittai BushlinAffiliated withDepartment of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine
  • , Noura Abul-HusnAffiliated withDepartment of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine
  • , Suresh P. AnnangudiAffiliated withDepartment of Chemistry and the Beckman Institute, University of Illinois at Urbana-Champaign
  • , Jonathan SweedlerAffiliated withDepartment of Chemistry and the Beckman Institute, University of Illinois at Urbana-Champaign
  • , Lakshmi A. DeviAffiliated withDepartment of Pharmacology and Systems Therapeutics, Mount Sinai School of Medicine

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Abstract

An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.

Key words

Neuroproteomics subcellular fractionation presynaptic terminal mass spectrometry quantitative proteomics differential isotopic labelling