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Epidermal Cells

Volume 585 of the series Methods in Molecular Biology pp 421-432

Date:

Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay

  • Lauren R. StrachanAffiliated withSan Francisco VA Medical Center, University of California
  • , Ruby GhadiallyAffiliated withSan Francisco VA Medical Center, University of California

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Abstract

Epidermal stem cells are of major importance for tissue homeostasis, wound repair, tumor initiation, and gene therapy. Here we describe an in vivo regeneration assay to test for the ability of keratinocyte progenitors to maintain an epidermis over the long-term in vivo. Limiting dilution analysis of epidermal repopulating units in this in vivo regeneration assay at sequential time points allows the frequency of short-term (transit amplifying cell) and long-term (stem cell) repopulating cells to be quantified.

Keywords

Stem cell Transit amplifying cell Epidermis Limiting dilution Skin Regeneration