Epidermal Cells

Volume 585 of the series Methods in Molecular Biology pp 253-260


Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization

  • Maria I. MorassoAffiliated withDevelopmental Skin Biology Section, NIH/NIAMS

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Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.

Key words

DNA RNA Gene expression Epidermis In situ hybridization