Protocol

Natural Killer Cell Protocols

Volume 612 of the series Methods in Molecular Biology pp 365-375

Date:

KIR Genotyping by Multiplex PCR-SSP

  • Smita KulkarniAffiliated withCancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick
  • , Maureen P. MartinAffiliated withCancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick
  • , Mary CarringtonAffiliated withCancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick

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Abstract

Diversity across KIR haplotypes stems from differences in numbers of inhibitory and activating receptors, as well as allelic polymorphism of individual genes. The KIR locus has undergone large expansions and contractions over time and is believed to be coevolving with genes encoding its HLA class I ligands located within the MHC locus. KIR and HLA compound genotypes have been associated with susceptibility to or protection from infectious, autoimmune, reproductive, and malignant disorders. We describe here a simple and reliable multiplex PCR-SSP (sequence-specific priming) method for relatively rapid and inexpensive genotyping of 15 KIR genes using standard agarose gel electrophoresis.

Key words

KIR genotyping NK cell KIR haplotypes multiplex PCR PCR-SSP