Protocol

Natural Killer Cell Protocols

Volume 612 of the series Methods in Molecular Biology pp 335-352

Date:

Functional Analysis of Human NK Cells by Flow Cytometry

  • Yenan T. BrycesonAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge
  • , Cyril FauriatAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge
  • , João M. NunesAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge
  • , Stephanie M. WoodAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge
  • , Niklas K. BjörkströmAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge
  • , Eric O. LongAffiliated withLaboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health
  • , Hans-Gustaf LjunggrenAffiliated withCenter for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital Huddinge

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Abstract

Natural killer (NK) cells are a subset of lymphocytes that contribute to innate immunity through cytokine secretion and target cell lysis. NK cell function is regulated by a multiplicity of activating and inhibitory receptors. The advance in instrumentation for multi-color flow cytometry and the generation of specific mAbs for different epitopes related to phenotypic and functional parameters have facilitated our understanding of NK cell responses. Here, we provide protocols for flow cytometric evaluation of degranulation and cytokine production by human NK cells from peripheral blood at the single-cell level. In addition to offering insight into the regulation of human NK cell responses, these techniques are applicable to the assessment of various clinical conditions, including the diagnosis of immunodeficiency syndromes.

Key words

Human natural killer cells immunophenotyping polychromatic flow cytometry CD107a lysosomal-associated membrane protein-1 chemokines IFN-γ MIP-1β TNF-α