Natural Killer Cell Protocols

Volume 612 of the series Methods in Molecular Biology pp 15-26


In Vitro Development of Human Killer–Immunoglobulin Receptor-Positive NK Cells

  • Frank CichockiAffiliated withUniversity of Minnesota Cancer Center
  • , Jeffrey S. MillerAffiliated withUniversity of Minnesota Cancer Center

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The in vitro culture system outlined in this chapter allows for the delineation of events that occur during the development of CD34+ hematopoietic precursor cells into mature KIR+ human NK cells. This system can also be utilized to study the effects of gene overexpression or knockdown on the process of NK cell differentiation through retroviral transduction and long-term culture. The necessary soluble factors and contact-dependent conditions for in vitro human NK cell development have been worked out in our laboratory over the past 16 years.

Key words

Human NK cell development lymphocyte differentiation from hematopoietic precursors