Volume 579 of the series Methods in Molecular Biology pp 3-18


Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics

  • Michael A. KiebishAffiliated withBiology Department, Boston College
  • , Xianlin HanAffiliated withDepartment of Internal Medicine, Washington University School of Medicine
  • , Thomas N. SeyfriedAffiliated withDepartment of Biology, Boston College

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Contamination from subcellular organelles and myelin has hindered attempts to characterize the lipidome of brain mitochondria. A high degree of mitochondrial purity is required for accurate measurements of the content and molecular species composition of mitochondrial lipids. We devised a discontinuous Ficoll and sucrose gradient procedure for the isolation and purification of brain mitochondria free from any detectable contamination. Shotgun lipidomics was used to analyze the lipid composition of the brain mitochondria. These procedures can be used to determine whether intrinsic lipid abnormalities underlie mitochondrial dysfunction associated with neurological and neurodegenerative diseases.

Key words

Nonsynaptic Synaptic Mitochondria Brain Lipidome