Protocol

Biofuels

Volume 581 of the series Methods in Molecular Biology pp 53-59

Date:

Genetic Transformation of Switchgrass

  • Yajun XiAffiliated withNorthwest A and F University
  • , Yaxin GeAffiliated withThe Samuel Roberts Noble Foundation
  • , Zeng-Yu WangAffiliated withThe Samuel Roberts Noble Foundation

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Summary

Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used as explants for inoculation with A. tumefaciens strain EHA105. Hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the selection agent. Calluses resistant to hygromycin were obtained after 5–6 weeks of selection. Soil-grown switchgrass plants were regenerated about 6 months after callus induction and Agrobacterium-mediated transformation.

Key words

Agrobacterium Biofuel crop Genetic transformation Panicum virgatum Switchgrass Transgenic plant