Methods in Molecular Biology Volume 546, 2009, pp 317-332
Date: 11 Mar 2009

In Toto Imaging of Embryogenesis with Confocal Time-Lapse Microscopy

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Microscopy has been one of the most direct and powerful tools since the beginning of biological research. Continued advances such as confocal and two-photon fluorescence microscopy and fluorescent proteins now make imaging useful at a variety of spatial scales (molecules, circuits, cells, tissues, and even whole embryos) and temporal scales (