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Tumor Biomarker Discovery

Volume 520 of the series Methods in Molecular Biology pp 21-38

Date:

Discovery of Antibody Biomarkers Using Protein Microarrays of Tumor Antigens Cloned in High Throughput

  • Madhumita ChatterjeeAffiliated withDepartment of Molecular Biology and Genetics, Barbara Ann Karmanos Cancer Institute, Wayne State University
  • , Jerzy WojciechowskiAffiliated withDepartment of Molecular Biology and Genetics, Barbara Ann Karmanos Cancer Institute, Wayne State University
  • , Michael A. TainskyAffiliated withDepartment of Molecular Biology and Genetics, Barbara Ann Karmanos Cancer Institute, Wayne State University

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Summary

Development of humoral and cellular immunity against self-cellular proteins in cancer patients is a phenomenal observation. The ability of immune system to sense the presence of the disease and to fight of the disease by generating autoantibodies against tumor antigens makes it a natural biosensor. Several screening technologies have been employed for the identification of tumor-specific antibodies in cancer patients. We have developed a multidimensional approach for the identification of diagnostic antigens that utilizes a combination of high-throughput antigen cloning and protein microarray-based serological detection of complex panels of antigens by exploiting the serum autoantibody repertoire directed toward tumor-associated antigens in cancer patients. Furthermore, validation of these antigens by different bioinformatics and biological approaches will reveal the diagnostic/prognostic utility of these antigens for personalized immunotherapy.

Keywords

Immune response Tumor biomarkers Autoantibodies Epitopes Protein microarrays Immunotherapy