Protocol

Tumor Biomarker Discovery

Volume 520 of the series Methods in Molecular Biology pp 143-150

Date:

High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays

  • Shannon L. ServossAffiliated withBiological Sciences Division, Pacific Northwest National Laboratory
  • , Rachel GonzalezAffiliated withBiological Sciences Division, Pacific Northwest National Laboratory
  • , Susan VarnumAffiliated withBiological Sciences Division, Pacific Northwest National Laboratory
  • , Richard C. ZangarAffiliated withBiological Sciences Division, Pacific Northwest National Laboratory Email author 

* Final gross prices may vary according to local VAT.

Get Access

Summary

Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.

Keywords

ELISA microarray Antibody microarray High throughput