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Cell-Based Assays for High-Throughput Screening

Volume 486 of the series Methods in Molecular Biology pp 1-12

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Introduction: Cell-Based Assays for High-Throughput Screening

  • W. Frank AnAffiliated withChemical Biology Platform, Broad Institute of Harvard and MIT
  • , Nicola J. TollidayAffiliated withChemical Biology Platform, Broad Institute of Harvard and MIT Email author 

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Summary

Cell-based assays represent approximately half of all high-throughput screens (HTS) currently performed. Here we review the history and status of HTS, and summarize some of the challenges and benefits associated with the use of cell-based assays in HTS, drawing upon themes that will reemerge in subsequent chapters in this book. Approaches for successful experimental design and execution of cell-based HTS are introduced, including strategies for assay development, implementation of primary and secondary screens, and target identification. In doing so, we hope to provide a comprehensive review of the cell-based HTS process and an introduction to the methodologies and techniques described in this book.

Key words

Assay development Cell-based assay High-content screening High-throughput screening Small-molecule screening Target identification