Cell Grafting and Labeling in Postimplantation Mouse Embryos
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Fate mapping experiments provide direct information on the differentiation pathways normally taken by cells or tissues during embryogenesis. Systematic analyses of the developmental fate of cell populations localized in different parts of the embryo enables the construction of fate maps. A comparison of the expression pattern of lineage-specific genes and the fate map allows the identification of precursor tissue for cell lineages well before definitive histogenesis takes place. The ability to trace the early lineage history of cells greatly facilitates the elucidation of the forces and processes that lead to the specification of cell lineages and the determination (or commitment) of cell fate. The knowledge of cell fate may also assist the interpretation of the phenotype of mutant embryos produced by either spontaneous mutation or gene knockout experiments.
This chapter describes the technical aspect of fate mapping the mouse embryo during gastrulation (6. ...
- Lawson KA, Meneses JJ, Pedersen RA (1991).Clonal analysis of epiblast fate during germ layer formation in the mouse embryo. Development 113:891–911.
- Quinlan GA, Williams EA, Tan S-S, Tam PPL (1995).Neuroectodermal fate of epiblast cells in the distal cap region of the mouse egg cylinder: implication for body plan organisation during early embryogenesis. Development 121:87–984.
- Parameswaran M, Tam PPL (1995) Regionalisation of cell fate and morphogenetic movement of the mesoderm during mouse gastrulation. Develop Genet 17:16–28. CrossRef
- Tam PPL, Beddington RSP (1987) The formation of mesodermal tissues in the mouse embryo during gastrulation and early organogenesis. Development 99:109–126.
- Tam PPL, Beddington RSP (1992) Establishing and organisation of germ layers in the gastrulating mouse embryo. In: Postimplantation development in the mouse, Ciba Foundation Symp. 165. Wiley, Chichester, UK, pp. 27–49.
- Tam PPL (1989) Regionalisation of mouse embryonic ectoderm: allocation of prospective ectodermal tissue during gastrulation. Development 107:55–67.
- Trainor PA, Tan S-S, Tam PPL (1994) Cranial paraxial mesoderm: regionalisation of cell fate and impact upon craniofacial development in mouse embryos. Development 120:2397–2408.
- Trainor PA, Tam PPL (1995) Cranial paraxial mesoderm and neural crest cells of the mouse embryo: co-distribution in the craniofacial mesenchyme but distinct segregation in branchial arches. Development 121:2569–2582.
- Davidson BP, Tsang TE, Khoo P-L, Gad JM, Tam PPL (2003) Introduction of cell markers into germ layer tissues of the mouse gastrula by whole embryo electroporation. Genesis 35:57–62. CrossRef
- Beddington RSP (1994) Induction of a second neural axis. Development 120:613– 620.
- Miura S, Mishina Y (2003) Whole-embryo culture of E5.5 mouse embryos: development to the gastrulation stage. Genesis 37:38–43. CrossRef
- Sturm K, Tam PPL (1992) Isolation and culture of whole postimplantation embryos and their germ layer derivatives. In: Wassarman P, DePamphilis M (eds) Methods in enzymology:. guide to techniques in mouse development. Academic Press, New York, pp. 164–190.
- Jacobson AG, Tam PPL (1982) Cephalic neurulation in the mouse embryo analysed by SEM and morphometry. Anat Rec 203:375–395. CrossRef
- Chan WY, Tam PPL (1988) A morphological and experimental study of the mesen-cephalic neural crest cells in the mouse embryo using wheat germ agglutinin-gold conjugates as the cell marker. Development 102:427–442.
- Serbedzija GN, Bronner-Fraser M, Fraser SE (1989) A vital dye analysis of timing and pathways of avian neural crest cell migration. Development 106:809–816.
- Kinder SJ, Tan S-S, Tam PPL (2000). Cell grafting and fate mapping of the early-somite-stage mouse embryo. In: Methods in molecular biology, vol.1, pp. 425–437, Developmental Biology Protocols, vol. 135.
- Cell Grafting and Labeling in Postimplantation Mouse Embryos
- Book Title
- Molecular Embryology
- Book Subtitle
- Methods and Protocols
- Book Part
- pp 47-70
- Print ISBN
- Online ISBN
- Series Title
- METHODS IN MOLECULAR BIOLOGY™
- Series Volume
- Series ISSN
- Humana Press
- Copyright Holder
- Humana Press
- Additional Links
- Industry Sectors
- Editor Affiliations
- 2. Department of Craniofacial Development, King's College London
- 3. MRC Centre for Developmental Neurobiology, King's College London
- Author Affiliations
- 4. Embryology Unit, Children's Medical Research Institute, University of Sydney, Sydney, Australia
- 5. Stowers Institute, Kansas City, Kansas, USA
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