Rat Genomics

Volume 597 of the series Methods in Molecular Biology pp 151-167


ENU Mutagenesis to Generate Genetically Modified Rat Models

  • Ruben van BoxtelAffiliated withHubrect Institute
  • , Michael N. GouldAffiliated withMcArdle Lab for Cancer Research, University of Wisconsin-Madison Email author 
  • , Edwin CuppenAffiliated withHubrecht Institute, NIOB KNAW
  • , Bart M. G. SmitsAffiliated withMcArdle Lab for Cancer Research, University of Wisconsin-Madison

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The rat is one of the most preferred model organisms in biomedical research and has been extremely useful for linking physiology and pathology to the genome. However, approaches to genetically modify specific genes in the rat germ line remain relatively scarce. To date, the most efficient approach for generating genetically modified rats has been the target-selected N-ethyl-N-nitrosourea (ENU) mutagenesis-based technology. Here, we describe the detailed protocols for ENU mutagenesis and mutant retrieval in the rat model organism.

Key words

ENU mutagenesis Rat strains Mutation discovery Mutation frequency Mutant Knockout models