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Molecular Endocrinology

Volume 590 of the series Methods in Molecular Biology pp 335-362

Date:

Transgenic Mouse Technology: Principles and Methods

  • T. Rajendra KumarAffiliated withDepartments of Molecular & Integrative Physiology, Pathology and Laboratory Medicine, and Institute of Maternal and Fetal Biolgoy, Center for Reproductive Sciences, University of Kansas Medical Center
  • , Melissa LarsonAffiliated withDepartment of Molecular and Integrative Physiology, University of Kansas Medical Center
  • , Huizhen WangAffiliated withDepartment of Molecular and Integrative Physiology, Center for Reproductive Sciences, University of Kansas Medical Center
  • , Jeff McDermottAffiliated withTransgenic and Gene-Targeting Institutional Facility, Universityof Kansas Medical Center
  • , Illya BronshteynAffiliated withTransgenic and Gene-Targeting Institutional Facility, Universityof Kansas Medical Center

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Abstract

Introduction of foreign DNA into the mouse germ line is considered a major technical advancement in the fields of developmental biology and genetics. This technology now referred to as transgenic mouse technology has revolutionized virtually all fields of biology and provided new genetic approaches to model many human diseases in a whole animal context. Several hundreds of transgenic lines with expression of foreign genes specifically targeted to desired organelles/cells/tissues have been characterized. Further, the ability to spatio-temporally inactivate or activate gene expression in vivo using the “Cre-lox” technology has recently emerged as a powerful approach to understand various developmental processes including those relevant to molecular endocrinology. In this chapter, we will discuss the principles of transgenic mouse technology, and describe detailed methodology standardized at our institute.

Key words

Transgene mouse embryo pseudopregnancy microinjection gene manipulation reporter genes integration of foreign DNA